In chemistry Raman spectroscopy is commonly used, this is because vibration information is specific to symmetry of molecules and the chemical bonds. Therefore, Raman spectroscopy provides a fingerprint by which the molecule can be known. For example, using infrared and Raman spectra, the vibration frequencies of Si3O3, Si3O2 and SiO were identified and assigned basing on their normal coordinating analyses. The technique is also used in studying changes in chemical bonding. This is when an enzyme acts on a substrate added on it.
During surgeries, Raman gas analyzers are used for real time monitoring of respiratory gas and anesthetic mixtures. This is done mainly in hospitals in deadly operations.
Other Widely used Applications of Spectra Physics Laser
When spontaneous Raman spectroscopy is in solid-state, it is used to; find the crystallographic direction of a sample, measure temperature and in characterizing materials. A particular solid material has quality phonon modes that can assist an experimenter identify it a particular solid material has quality phonon modes that can assist an experimenter identify it using single molecules.
Low frequency excitations such as superconducting gap, magnons and plasmons can be observed using Raman spectroscopy. Can be used to observe other low frequency excitations of the solid, such as plasmons, magnons, and superconducting gap excitations. The spontaneous Raman indicator provides information on the population of a particular phonon mode in the ratio between the up shift and the downshift intensity.
Spectra physics Laser Polarization
When a Raman is scattered using an anisotropic crystal, it gives information on the crystal orientation. If the crystal structure is known, one can find the orientation of the crystal using the polarization of the Raman scattered light with respect to the polarization of the laser light and the crystal.
With applied stress, Raman active fibers, like carbon and aramid have vibration modes that illustrate a shift in Raman frequency. Other fibers that exhibit similar shifts include Polypropylene. Fibers also exhibit similar shifts. To evaluate the diameter of carbon nanotubes, the radial breathing mode is a usually used. Raman microscope analyses the nanowires to better the understanding of the composite structure.
Micro spectroscopy in Spectra physics Laser
Raman spectroscopy offers quite a lot of merits for microscopic analysis. Specimens do not need to be sectioned or fixed since the spectroscopy uses the scattering technique. And because Raman spectra can be collected from a extremely small volumes they allow the identification of species present.
Raman spectroscopy is appropriate for the microscopic assessment of cells, minerals, proteins, materials such as polymers and ceramics, and forensic trace evidence. This is because water does not generally interfere with Raman spectral analysis.
A Raman microscope has a standard ocular microscope, a monochromatic, an excitation laser, and a sensitive detector. FT-Raman also has been commonly used with microscopes. Ultraviolet microscopes and enhanced optics must be used when a Ultraviolet laser source is in use for Raman micro spectroscopy.